phosphorylated akt ser473 (Cell Signaling Technology Inc)
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Phosphorylated Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 32625 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 32625 article reviews
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Incubation:Article Title: Ivabradine Attenuates Experimental Hepatic Fibrosis by Modulating Inflammatory and Apoptotic Signaling Pathways Article Snippet: .. The blots were then incubated overnight at 4 °C with gentle agitation using the primary antibodies PI3K p85 (Cell Signaling, Danvers, MA, USA, #4292, 85 kDa, 1:1000), phosphorylated PI3K p85/p55 (Tyr467/Tyr199/Tyr464) (Invitrogen, Carlsbad, CA, USA, #PIPA5121306, 85 kDa, 1:1000), AKT (Cell Signaling, #9272, 60 kDa, 1:1000), Article Title: TAT-PBX1 Reverses Hyperglycemia Through β-Cell Regeneration and Functional Restoration in an STZ-Induced Diabetic Model Article Snippet: .. Membranes were then incubated overnight at 4 °C with primary antibodies: PBX1 (HA721303, dilution ratio 1:1000, Huabio, Hangzhou, China), Caspase 3 (AF6311, dilution ratio 1:1000, Affinity, Shanghai, China), Cleaved Caspase 3 (9661, dilution ratio 1:1000, CST, Danvers, MA, USA), GK (DF8549, dilution ratio 1:1000, Affinity, China), Pancreatic and Duodenal Homeobox 1 (PDX1, 20989-1-AP, dilution ratio 1:1000, Proteintech, Wuhan, China), p53-binding protein 1 (53BP1, ab36823, dilution ratio 1:1000, Abcam, Cambridge, UK), PARP1 (9532, dilution ratio 1:1000, CST, USA), γ-H2A.X (2577, dilution ratio 1:1000, CST, USA), Insulin Receptor Substrate 1 (IRS1, 17509-1-AP, dilution ratio 1:1000, Proteintech, China), Phosphoinositide 3-kinase regulatory subunit p85 (PI3K p85, T40115 , dilution ratio 1:1000, Abmart, Shanghai, China), Phosphorylated PI3K p85 (p-PI3K p85, T40116 , dilution ratio 1:1000, Abmart, China), AKT (9272, dilution ratio 1:1000, CST, USA), Article Title: Ivabradine Attenuates Experimental Hepatic Fibrosis by Modulating Inflammatory and Apoptotic Signaling Pathways. Article Snippet: .. The blots were then incubated overnight at 4 ◦C with gentle agitation using the primary antibodies PI3K p85 (Cell Signaling, Danvers, MA, USA, #4292, 85 kDa, 1:1000), phosphorylated PI3K p85/p55 (Tyr467/Tyr199/Tyr464) (Invitrogen, Carlsbad, CA, USA, #PIPA5121306, 85 kDa, 1:1000), AKT (Cell Signaling, #9272, 60 kDa, 1:1000), Article Title: TAT-PBX1 Reverses Hyperglycemia Through β-Cell Regeneration and Functional Restoration in an STZ-Induced Diabetic Model. Article Snippet: .. Membranes were then incubated overnight at 4 ◦C with primary antibodies: PBX1 (HA721303, dilution ratio 1:1000, Huabio, Hangzhou, China), Caspase 3 (AF6311, dilution ratio 1:1000, Affinity, Shanghai, China), Cleaved Caspase 3 (9661, dilution ratio 1:1000, CST, Danvers, MA, USA), GK (DF8549, dilution ratio 1:1000, Affinity, China), Pancreatic and Duodenal Homeobox 1 (PDX1, 20989-1-AP, dilution ratio 1:1000, Proteintech, Wuhan, China), p53-binding protein 1 (53BP1, ab36823, dilution ratio 1:1000, Abcam, Cambridge, UK), PARP1 (9532, dilution ratio 1:1000, CST, USA), γ-H2A.X (2577, dilution ratio 1:1000, CST, USA), Insulin Receptor Substrate 1 (IRS1, 17509-1-AP, dilution ratio 1:1000, Proteintech, China), Phosphoinositide 3-kinase regulatory subunit p85 (PI3K p85, T40115, dilution ratio 1:1000, Abmart, Shanghai, China), Phosphorylated PI3K p85 (p-PI3K p85, T40116, dilution ratio 1:1000, Abmart, China), AKT (9272, dilution ratio 1:1000, CST, USA), Article Title: Poly(ADP-ribose) Polymerase Inhibitor Talazoparib Inhibits DNA Damage Repair in Breast Cancer Cell Lines With or Without BRCA1/2 Mutation. Article Snippet: The cells were collected and fractionated using a Subcellular Protein Fraction Kit for Cultured Cells (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions. .. Separated proteins were transferred onto nitrocellulose membranes, and the blots were incubated overnight at 4°C with the following primary antibodies: PARP1 (sc-8007; Santa Cruz Biotechnology, Santa Cruz, USA), Lamin B1(sc-374015), Chk1 (sc-7898), Chk2 (sc-5278), Cyclin B1 (sc-245), TopBP1 (sc-271043), Rad9 (sc-8324), Hus1 (sc-30543), Rad51 (sc-377467), Gentle:Article Title: Ivabradine Attenuates Experimental Hepatic Fibrosis by Modulating Inflammatory and Apoptotic Signaling Pathways Article Snippet: .. The blots were then incubated overnight at 4 °C with gentle agitation using the primary antibodies PI3K p85 (Cell Signaling, Danvers, MA, USA, #4292, 85 kDa, 1:1000), phosphorylated PI3K p85/p55 (Tyr467/Tyr199/Tyr464) (Invitrogen, Carlsbad, CA, USA, #PIPA5121306, 85 kDa, 1:1000), AKT (Cell Signaling, #9272, 60 kDa, 1:1000), Article Title: Ivabradine Attenuates Experimental Hepatic Fibrosis by Modulating Inflammatory and Apoptotic Signaling Pathways. Article Snippet: .. The blots were then incubated overnight at 4 ◦C with gentle agitation using the primary antibodies PI3K p85 (Cell Signaling, Danvers, MA, USA, #4292, 85 kDa, 1:1000), phosphorylated PI3K p85/p55 (Tyr467/Tyr199/Tyr464) (Invitrogen, Carlsbad, CA, USA, #PIPA5121306, 85 kDa, 1:1000), AKT (Cell Signaling, #9272, 60 kDa, 1:1000), Hemagglutination Assay:Article Title: Novel NOTCH2-NTRK1 fusion confers osimertinib resistance in EGFR-mutant non-small cell lung cancer by interacting with EGFR Article Snippet: BEAS-2B cells were grown in a DMEM medium, while H1650 and H1975 cells were cultured in an RPMI-1640 medium, plus with 10 %FBS, which were sourced from HyClone. .. Antibodies for total AKT (#4691), phosphorylated AKT (Ser473) (#4060), total ERK (#4695), phosphorylated ERK (Thr202/Tyr204) (#4370), HA (C29F4) (#3724), EGFR (D38B1) (#4267), phosphorylated EGFR (Thr669) (D2F1) (#8808), Met (D1C2) (#8198) and phosphorylated TRKA (Tyr490) (#4619) were purchased from Cell Signaling Technology. .. The anti-GAPDH antibody (#47724) was obtained from Santa Cruz Biotechnology. Article Title: Novel NOTCH2-NTRK1 fusion confers osimertinib resistance in EGFR-mutant non-small cell lung cancer by interacting with EGFR. Article Snippet: BEAS-2B cells were grown in a DMEM medium, while H1650 and H1975 cells were cultured in an RPMI-1640 medium, plus with 10 %FBS, which were sourced from HyClone. .. Antibodies for total AKT (#4691), phosphorylated AKT (Ser473) (#4060), total ERK (#4695), phosphorylated ERK (Thr202/Tyr204) (#4370), HA (C29F4) (#3724), EGFR (D38B1) (#4267), phosphorylated EGFR (Thr669) (D2F1) (#8808), Met (D1C2) (#8198) and phosphorylated TRKA (Tyr490) (#4619) were purchased from Cell Signaling Technology. .. The anti-GAPDH antibody (#47724) was obtained from Santa Cruz Biotechnology. Cell-Signaling:Article Title: Novel NOTCH2-NTRK1 fusion confers osimertinib resistance in EGFR-mutant non-small cell lung cancer by interacting with EGFR Article Snippet: BEAS-2B cells were grown in a DMEM medium, while H1650 and H1975 cells were cultured in an RPMI-1640 medium, plus with 10 %FBS, which were sourced from HyClone. .. Antibodies for total AKT (#4691), Article Title: Novel NOTCH2-NTRK1 fusion confers osimertinib resistance in EGFR-mutant non-small cell lung cancer by interacting with EGFR. Article Snippet: BEAS-2B cells were grown in a DMEM medium, while H1650 and H1975 cells were cultured in an RPMI-1640 medium, plus with 10 %FBS, which were sourced from HyClone. .. Antibodies for total AKT (#4691), Article Title: Poly(ADP-ribose) Polymerase Inhibitor Talazoparib Inhibits DNA Damage Repair in Breast Cancer Cell Lines With or Without BRCA1/2 Mutation. Article Snippet: The cells were collected and fractionated using a Subcellular Protein Fraction Kit for Cultured Cells (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions. .. Separated proteins were transferred onto nitrocellulose membranes, and the blots were incubated overnight at 4°C with the following primary antibodies: PARP1 (sc-8007; Santa Cruz Biotechnology, Santa Cruz, USA), Lamin B1(sc-374015), Chk1 (sc-7898), Chk2 (sc-5278), Cyclin B1 (sc-245), TopBP1 (sc-271043), Rad9 (sc-8324), Hus1 (sc-30543), Rad51 (sc-377467), Recombinase Polymerase Amplification:Article Title: Poly(ADP-ribose) Polymerase Inhibitor Talazoparib Inhibits DNA Damage Repair in Breast Cancer Cell Lines With or Without BRCA1/2 Mutation. Article Snippet: The cells were collected and fractionated using a Subcellular Protein Fraction Kit for Cultured Cells (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions. .. Separated proteins were transferred onto nitrocellulose membranes, and the blots were incubated overnight at 4°C with the following primary antibodies: PARP1 (sc-8007; Santa Cruz Biotechnology, Santa Cruz, USA), Lamin B1(sc-374015), Chk1 (sc-7898), Chk2 (sc-5278), Cyclin B1 (sc-245), TopBP1 (sc-271043), Rad9 (sc-8324), Hus1 (sc-30543), Rad51 (sc-377467), Mutagenesis:Article Title: Poly(ADP-ribose) Polymerase Inhibitor Talazoparib Inhibits DNA Damage Repair in Breast Cancer Cell Lines With or Without BRCA1/2 Mutation. Article Snippet: The cells were collected and fractionated using a Subcellular Protein Fraction Kit for Cultured Cells (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions. .. Separated proteins were transferred onto nitrocellulose membranes, and the blots were incubated overnight at 4°C with the following primary antibodies: PARP1 (sc-8007; Santa Cruz Biotechnology, Santa Cruz, USA), Lamin B1(sc-374015), Chk1 (sc-7898), Chk2 (sc-5278), Cyclin B1 (sc-245), TopBP1 (sc-271043), Rad9 (sc-8324), Hus1 (sc-30543), Rad51 (sc-377467), |

